Journal: Frontiers in Oncology

Article Title: IL-1β enhances cell viability and decreases 5-FU sensitivity in novel colon cancer cell lines derived from African American patients

doi: 10.3389/fonc.2022.1010380

Figure Lengend Snippet: Differential gene expression of ILB , MAPK , and NFKB related genes between AA and CA colon cancer cell lines. Cell lines have been grouped based on their microsatellite status in MSS (CHTN-06 vs. HT-29) and MSI (SB-521 vs. HCT-116). Graphical representation of fold change of genes compared between cell lines.

Article Snippet: Therefore, for this study, we grouped the cell lines as MSS (CHTN-06 and HT-29) and MSI (SB-521 and HCT-116) and evaluated their response to 5-FU treatment.

Techniques: Gene Expression

Journal: Frontiers in Oncology

Article Title: IL-1β enhances cell viability and decreases 5-FU sensitivity in novel colon cancer cell lines derived from African American patients

doi: 10.3389/fonc.2022.1010380

Figure Lengend Snippet: IL-1β decreases sensitivity to 5-FU treatment. Cell viability following treatment with 5-FU, in the presence or absence of IL-1β, was determined by MTS assay. Cells were seeded in media containing 10 ng/ml of IL-1β and then treated with increasing concentrations of 5-FU and incubated for 72 hours. (A) CHTN-06 AA cell lines, MSS; HT-29 CA cell line, MSS; (B) SB-521 AA cell line, MSI; HCT 116 CA cell line, MSI. Data are representative of three independent experiments. Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Therefore, for this study, we grouped the cell lines as MSS (CHTN-06 and HT-29) and MSI (SB-521 and HCT-116) and evaluated their response to 5-FU treatment.

Techniques: MTS Assay, Incubation

Journal: Frontiers in Oncology

Article Title: IL-1β enhances cell viability and decreases 5-FU sensitivity in novel colon cancer cell lines derived from African American patients

doi: 10.3389/fonc.2022.1010380

Figure Lengend Snippet: IL-1β decreases 5-FU-induced apoptosis in MSS cell lines. Apoptosis detection by Annexin V/PI staining via flow cytometry following treatment with different concentrations of 5-FU (0, 2.5, 5 mM), in the presence or absence of IL-1β. Representative images of flow cytometry data for CHTN-06 (A) and HT-29 (C) ; Percentage of combined early apoptotic (Annexin V+) and late apoptotic (Annexin V+/PI+) cells for CHTN-06 (B) and HT-29 (D) cell lines. Data are representative of three independent experiments. Error bars represent SEM. *p<0.05, **p<0.01.

Article Snippet: Therefore, for this study, we grouped the cell lines as MSS (CHTN-06 and HT-29) and MSI (SB-521 and HCT-116) and evaluated their response to 5-FU treatment.

Techniques: Staining, Flow Cytometry

Journal: Frontiers in Oncology

Article Title: IL-1β enhances cell viability and decreases 5-FU sensitivity in novel colon cancer cell lines derived from African American patients

doi: 10.3389/fonc.2022.1010380

Figure Lengend Snippet: IL-1Ra counteracts IL-1β effects on 5-FU sensitivity. Cell viability following treatment with 5-FU alone, in presence of IL-1β, or in combination with IL-1Ra was determined by MTS assay. Cells were seeded in media, in the presence or absence of 10 ng/ml of IL-1β and then treated with 5-FU alone or in combination with IL-1Ra, and incubated for 72 hours. (A) MSS cell lines CHTN-06 and HT-29; (B) MSI cell lines SB-521 and HCT-116. Data are representative of three independent experiments. Error bars represent SEM. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Therefore, for this study, we grouped the cell lines as MSS (CHTN-06 and HT-29) and MSI (SB-521 and HCT-116) and evaluated their response to 5-FU treatment.

Techniques: MTS Assay, Incubation